To easily, surely, accurately and efficiently carry out the supply work of paper cylinder seedling rows to a transplanter while reducing the physical burden of a seedling supplier.

The paper cylinder seedling group separator includes a fixed frame body KW1 including a contact plate 12 supported by the front ends of upper and lower support pieces 11, and a vertical handle 13 supported by the rear ends of the upper and lower support pieces 11; a right side turning frame body RW1R with its front end turnably supported to right side support pieces 15 fixed to the contact plate 12, with its rear end supporting a right side horizontal handle 23 and with its right side center horizontal shaft 21 provided with a large number of curved right side engaging needles 22 inserted therein; and a left side turning frame body RW1L with its front end turnably supported to left side support pieces 16 fixed to the contact plate 12, with its rear end supporting a left side horizontal handle 26 and with its left side center horizontal shaft 24 provided with a large number of curved left side engaging needles 25 inserted therein.