To recovery particles collected in an electrode by a simple operation.

The particle separation apparatus 1 is provided with a storing vessel 6 to store a particle suspended liquid containing a plurality of types of particles, of which the dielectrophoresis characteristics are different, an electrode 3 arranged within the storing vessel 6, a voltage supply part 5 which applies voltage to the electrode 3 so that non-uniform electric field in which the potential gradient varies in accordance with the distance from the electrode 3 may be generated, a suction tube 7 which consists of an electrical insulation material to which a suction mouth 7a is arranged at the proximity of the electrode 3 and a suction means 10 to suck the inside of the suction tube 7.