Title:
PCR METHOD AND PCR KIT
Document Type and Number:
Japanese Patent JP2016042830
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a PCR method and a PCR kit which have higher detection accuracy and are simple.SOLUTION: In a PCR method of the present invention, a sample including: a primer set including a first primer and a second primer; a template amplified by the primer set; a first probe in which a bulge structure is lost by forming a double strand together with the first primer, and the bulge structure is formed by dissociating from the double strand formed together with the first primer; and a bulge structure binding molecule which emits a signal by being bound to the bulge structure, is subjected to PCR reaction.SELECTED DRAWING: Figure 1
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Inventors:
NAKATANI KAZUHIKO
SAKAMOTO FUMIE
SAKAMOTO FUMIE
Application Number:
JP2014169900A
Publication Date:
April 04, 2016
Filing Date:
August 22, 2014
Export Citation:
Assignee:
UNIV OSAKA
International Classes:
C12N15/09; C12Q1/68
Domestic Patent References:
| JP2011182763A | 2011-09-22 |
Foreign References:
| WO2008026582A1 | 2008-03-06 | |||
| WO2013133402A1 | 2013-09-12 |
Other References:
兪改改 ほか: "DNA-シトシンバルジ構造に結合する蛍光分子を用いた蛍光増加型PCRの開発", 日本化学会第93春季年会(2013)講演予稿集III, vol. 2013年3月8日発行, JPN6015046037, pages p. 925
Attorney, Agent or Firm:
Harakenzo world patent & trademark
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