Title:
光学検出装置の性能確認方法およびそれに用いる標準試薬
Document Type and Number:
Japanese Patent JP5255439
Kind Code:
B2
Abstract:
A method is provided in which with respect to an optical detection apparatus including an optical detection unit and a temperature control unit, whether optical signal detection and temperature control are performed accurately, i.e. the performance thereof, can be verified simply with high reliability. With respect to an optical detection apparatus including an optical detection unit for detecting an optical signal of a sample and a temperature control unit for controlling temperature of the sample, the optical signal detection performance and temperature control performance are verified by the following method. First, a standard sample containing a nucleic acid sequence and a strand complementary thereto that have a known optical signal intensity and Tm value is provided, the temperature of the standard sample is increased or decreased with the temperature control unit, and optical signal intensity of the standard sample is measured with the detection unit. On the other hand, the melting temperature of the standard sample is determined from a change in the optical signal intensity accompanying a change in the temperature. The measured optical signal intensity and melting temperature of the standard sample are compared to the known optical signal intensity and melting temperature of the standard sample, respectively, and thereby it is verified whether the optical signal detection performance of the detection unit and the temperature control performance of the temperature control unit are accurate.
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Inventors:
Hirai Mitsuharu
Toshiya Hosomi
Yoshinaga Yuki
Toshiya Hosomi
Yoshinaga Yuki
Application Number:
JP2008524235A
Publication Date:
August 07, 2013
Filing Date:
December 25, 2007
Export Citation:
Assignee:
Arkray Co., Ltd.
International Classes:
G01N21/64; C12M1/00; C12N15/09; C12Q1/68; G01N21/78; G01N33/53; G01N35/00
Domestic Patent References:
| JP2005058107A | 2005-03-10 | |||
| JPH10332701A | 1998-12-18 | |||
| JP2002119291A | 2002-04-23 | |||
| JP2005058107A | 2005-03-10 |
Other References:
JPN6013001445; Ａｒａｓｈ Ｄｏｄｇｅ ｅｔ ａｌ．: 'Ａ Ｍｉｃｒｏｆｌｕｉｄｉｃ Ｐｌａｔｆｏｒｍ Ｕｓｉｎｇ Ｍｏｌｅｃｕｌａｒ Ｂｅａｃｏｎ-Ｂａｓｅｄ Ｔｅｍｐｅｒａｔｕｒｅ Ｃａｌｉｂｒａｔｉｏｎ ｆｏｒ Ｔｈｅｒｍａｌ Ｄｅｈｙｂｒｉｄｉｚａ' Ａｎａｌ． Ｃｈｅｍ． ７６ （６）, 2004, １７７８-１７８７
JPN7013000146; Ｃｈｒｉｓｔｏｆｆｅｒ Ｎｅｌｌａｋｅｒ ｅｔ ａｌ．: 'Ｍｏｌｅｃｕｌａｒ Ｂｅａｃｏｎ．Ｂａｓｅｄ Ｔｅｍｐｅｒａｔｕｒｅ Ｃｏｎｔｒｏｌ ａｎｄ Ａｕｔｏｍａｔｅｄ Ａｎａｌｙｓｅｓ ｆｏｒ Ｉｍｐｒｏｖｅｄ Ｒｅｓｏｌｕｔｉｏｎ ｏｆ Ｍｅｌｔｉｎｇ' Ｃｌｉｎｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｖｏｌ．５３， Ｎｏ．１, 2007, ９８-１０３
JPN6013001445; Ａｒａｓｈ Ｄｏｄｇｅ ｅｔ ａｌ．: 'Ａ Ｍｉｃｒｏｆｌｕｉｄｉｃ Ｐｌａｔｆｏｒｍ Ｕｓｉｎｇ Ｍｏｌｅｃｕｌａｒ Ｂｅａｃｏｎ-Ｂａｓｅｄ Ｔｅｍｐｅｒａｔｕｒｅ Ｃａｌｉｂｒａｔｉｏｎ ｆｏｒ Ｔｈｅｒｍａｌ Ｄｅｈｙｂｒｉｄｉｚａ' Ａｎａｌ． Ｃｈｅｍ． ７６ （６）, 2004, １７７８-１７８７
JPN7013000146; Ｃｈｒｉｓｔｏｆｆｅｒ Ｎｅｌｌａｋｅｒ ｅｔ ａｌ．: 'Ｍｏｌｅｃｕｌａｒ Ｂｅａｃｏｎ．Ｂａｓｅｄ Ｔｅｍｐｅｒａｔｕｒｅ Ｃｏｎｔｒｏｌ ａｎｄ Ａｕｔｏｍａｔｅｄ Ａｎａｌｙｓｅｓ ｆｏｒ Ｉｍｐｒｏｖｅｄ Ｒｅｓｏｌｕｔｉｏｎ ｏｆ Ｍｅｌｔｉｎｇ' Ｃｌｉｎｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｖｏｌ．５３， Ｎｏ．１, 2007, ９８-１０３
JPN6013001445; Ａｒａｓｈ Ｄｏｄｇｅ ｅｔ ａｌ．: 'Ａ Ｍｉｃｒｏｆｌｕｉｄｉｃ Ｐｌａｔｆｏｒｍ Ｕｓｉｎｇ Ｍｏｌｅｃｕｌａｒ Ｂｅａｃｏｎ-Ｂａｓｅｄ Ｔｅｍｐｅｒａｔｕｒｅ Ｃａｌｉｂｒａｔｉｏｎ ｆｏｒ Ｔｈｅｒｍａｌ Ｄｅｈｙｂｒｉｄｉｚａ' Ａｎａｌ． Ｃｈｅｍ． ７６ （６）, 2004, １７７８-１７８７
Attorney, Agent or Firm:
Koichiro Tsujimaru
Yumi Nakayama
Reiko Yoshida
Isaji So
Yumi Nakayama
Reiko Yoshida
Isaji So