熱安定性が向上したＰｈｉ２９ＤＮＡポリメラーゼ突然変異体及びそのシーケンシングにおける応用

Title:

熱安定性が向上したＰｈｉ２９ＤＮＡポリメラーゼ突然変異体及びそのシーケンシングにおける応用

Document Type and Number:

Japanese Patent JP7256280

Kind Code:

B2

Abstract:

Provided are a Phi29 DNA polymerase mutant with improved thermal stability and an use thereof in sequencing. The phi29 DNA polymerase mutant is represented by A) or B) below: the DNA polymerase mutant represented by the A) is a protein having DNA polymerase activity that is obtained by modifying at least one amino acid residue(s) in the following six sites in a phi29 DNA polymerase amino acid sequence: the 97-th, 123-th, 217-th, 224-th, 515-th, and 474-th sites; the DNA polymerase mutant represented by the B) is a protein having DNA polymerase activity that is derived from the A) by adding a tag sequence to a terminal of the amino acid sequence of the protein represented by the A).

More Like This:

WO/2000/060061	TRANSGENIC PLANT RESISTANT TO MYCOTOXINS AND METHODS
WO/2015/167282	A NOVEL METHOD FOR GLYCOSYLATION OF GINSENOSIDE USING A GLYCOSYLTRANSFERASE DERIVED FROM PANAX GINSENG
WO/1998/020114	NOVEL RECEPTOR TYROSINE KINASES

Inventors:

Zhang Zhou Go
Liu delight
Chung Yue
Shutama-kun
Summer army
Ryo Tou
Xu Chong Feng
Chapter sentence

Application Number:

JP2021546026A

Publication Date:

April 11, 2023

Filing Date:

October 11, 2018

Export Citation:

Click for automatic bibliography generation Help

Assignee:

深▲せん▼華大生命科学研究院

International Classes:

C12N15/54; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12; C12N15/63; C12Q1/6844; C12Q1/6869; C40B50/06

Foreign References:

US20130217007
US20140322759
US20140094374

Other References:

Protein Eng. Des. Sel., 2016, 29(12), pp.617-628

Attorney, Agent or Firm:

Sonoda & Kobayashi Patent Attorneys Corporation

Previous Patent: Method, device, computer program and electronic device for grouping operating units

Next Patent: WASTE WATER PURIFYING METHOD AND TANK