PHOSPHOLAMBAN-TARGETED MODIFIED RNA APTAMER

Title:

PHOSPHOLAMBAN-TARGETED MODIFIED RNA APTAMER

Document Type and Number:

Japanese Patent JP2013162771

Kind Code:

A

Abstract:

To provide a therapeutic and preventive medicines for heart failure which are not accompanied by genetic manipulation or gene expression, by providing a phospholamban-binding substance, and a therapeutic medicine and a preventive medicine for heart failure, which correct abnormality in the dynamic state of Ca²⁺ in myocardial cells in which the substance exists.

By the use of a fusion protein including the cytoplasmic domain of phospholamban as a target, a phospholamban-targeted modified mRNA aptamer is identified with the SELEX method. The aptamer, its deletion mutant, and the like, is found to possess the stability in blood, and, by binding to phospholamban to release suppression of the SERCA activity of the phospholamban, and to enhance the SERCA (Ca²⁺ pump ATPase of the cardiac sarcoplasmic reticulum) activity, and to enhance the contraction and relaxation capacity of myocardial cells. The phospholamban-modified mRNA aptamer can be used as a therapeutic and preventive medicines for heart failure.

Inventors:

INUI MAKOTO
TANAKA TAKAE
SAKAI DAIKI
IKEDA YASUHIRO
MATSUZAKI MASUNORI

Application Number:

JP2012028555A

Publication Date:

August 22, 2013

Filing Date:

February 13, 2012

Export Citation:

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Assignee:

UNIV YAMAGUCHI

International Classes:

C12N15/115; A61K31/7105; A61K31/7125; A61K48/00; A61P9/00; A61P9/04; A61P43/00; C12N9/99; C12N15/09; C12N9/16

Domestic Patent References:

JP2009189292A	2009-08-27
JP2008512097A	2008-04-24
JP2007527246A	2007-09-27

Foreign References:

WO2010143714A1

2010-12-16

Attorney, Agent or Firm:

Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Large High Toshiko
High Kazuya Tsu
Makoto Horiuchi

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