Title:
生体分子の検出および単離のための光分解性の反応剤ならびにコンジュゲート
Document Type and Number:
Japanese Patent JP4147230
Kind Code:
B2
Abstract:
This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
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Inventors:
Kennis, Jay, Ras Schild
Sanya, M, Suna
Jazi, Alenik
Sanya, M, Suna
Jazi, Alenik
Application Number:
JP2005174413A
Publication Date:
September 10, 2008
Filing Date:
June 14, 2005
Export Citation:
Assignee:
Trustees, Av, Beaustan, Universati
International Classes:
C12Q1/68; G01N33/531; A61K9/127; A61K38/00; A61K38/17; A61K39/00; A61K41/00; A61K47/48; A61K49/00; A61K51/00; A61P31/04; A61P31/12; B01J19/12; C07C205/45; C07C205/57; C07D207/404; C07D207/46; C07D311/16; C07D405/12; C07D495/04; C07H19/04; C07H19/06; C07H19/16; C07H21/00; C07H21/04; C07K1/107; C07K1/13; C07K14/00; C07K14/02; C07K14/03; C07K14/085; C07K14/155; C07K14/28; C07K14/465; C07K19/00; C12N15/09; C12P21/00; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/25; C12Q1/70; G01N33/532; C07C205/00; C07C205/06; C07C205/07; C07D207/40; C07D235/02
Domestic Patent References:
| JP10500409A |
Other References:
ANALYTICAL BIOCHEMISTRY,1994年,Vol.218,p.330-337
Annu. Rev. Biophys. Biophys. Chem.,,1989年,Vol.18,p.239-270
J. Org. Chem.,1988年,Vol.53,p.1966-1969
BIOCHEMISTRY,1992年,Vol.31,p.5500-550
Annu. Rev. Biophys. Biophys. Chem.,,1989年,Vol.18,p.239-270
J. Org. Chem.,1988年,Vol.53,p.1966-1969
BIOCHEMISTRY,1992年,Vol.31,p.5500-550
Attorney, Agent or Firm:
Yuzo Sanada
Ohara Shizuo
Ohara Shizuo