Title:
PLASMID AND PRODUCTION THEREOF
Document Type and Number:
Japanese Patent JPH01132386
Kind Code:
A
Abstract:
PURPOSE:To obtain a plasmid including autoreproduction of Saccharomycopsis lipolytica, by separating a plasmid from a leucine-demanding Saccharomyces cerevisiae transformed with a gene library. CONSTITUTION:An ampicillin-resistant plasmid is scissored with a restriction enzyme, Leu 2 gene range of Saccharomycopsis lipolytica is isolated, Escherichia coli vector is scissored with a restriction enzyme and connected to the isolated range. The gene library of Saccharomycopsis lipolytica is reproduced by using the connected plasmid. Then leucine-demanding Saccharomycopsis lipolytica as a host is transformed with the gene library. A plasmid is separated from the prepared transformant, Escherichia coli is transformed and the ampicillin- resistant plasmid is separated.
Inventors:
NAGASAKI TOMOHISA
UCHIDA KOJI
UCHIDA KOJI
Application Number:
JP29069687A
Publication Date:
May 24, 1989
Filing Date:
November 19, 1987
Export Citation:
Assignee:
ORIENTAL YEAST CO LTD
NISSHIN FLOUR MILLING CO
NISSHIN FLOUR MILLING CO
International Classes:
C12N15/09; C12N15/00; C12N15/81; C12R1/645; (IPC1-7): C12N15/00
Attorney, Agent or Firm:
Toda Chikio