Title:
多型検出法
Document Type and Number:
Japanese Patent JP2008533983
Kind Code:
A
Abstract:
A method for detecting the presence or absence of a polymorphism at a particular position within a nucleic acid sequence in a sample, said method comprising i) annealing a pair of probes to said nucleic acid sequence, said probes being designed such that they anneal adjacent each other, and only completely anneal to one form of the sequence, in a reaction mixture which is substantially free of ATP; ii) ligating together any completely annealed pairs of probes in the presence of NAD and a nucleic acid ligase which uses NAD as a substrate/ iii) phosphorylating any AMP released by a ligation event to ATP, and iv) detecting ATP, in particular using a bioluminescent reaction, in the resultant reaction mixture. Kits for conducting this method are also described and claimed.
Inventors:
Squirrel, David James
Application Number:
JP2008502464A
Publication Date:
August 28, 2008
Filing Date:
March 22, 2006
Export Citation:
Assignee:
Enigma Diagnostics Limited
International Classes:
C12Q1/68; C12N15/09
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe