PREPARATION OF SULFIDE AND AMINO ACID LABELED WITH 35S

Title:

PREPARATION OF SULFIDE AND AMINO ACID LABELED WITH 35S

Document Type and Number:

Japanese Patent JPH05229964

Kind Code:

A

Abstract:

PURPOSE: To provide a simple and easy method to produce a sulfide and amino acid or its deriv. labeled with 35S when these compds. are required.

CONSTITUTION: The production of a sulfide having high specific activity and labeled with 35S includes a step to prepare a reducing mixture containing hydriodic acid and hypophosphorous acid, a step to pretreat the mixture to remove sulfate ions present in the mixture, a step to reduce a sulfate labeled with 35S with the mixture, and a step to collect the sulfide labeled with 35S obtd. by the reduction. The sulfide labeled with 35S thus obtd. is used as a substrate in an enzyme reaction to produce an amino acid labeled with 35S or its deriv.

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Inventors:

KUROODO BURE
JIYANNPIEERU NOERU
MISHIERU RIBUA

Application Number:

JP19615692A

Publication Date:

September 07, 1993

Filing Date:

June 30, 1992

Export Citation:

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Assignee:

COMMISSARIAT ENERGIE ATOMIQUE

International Classes:

C12P11/00; C01B17/16; C01B17/20; C07B59/00; C07C323/58; C12P13/12; (IPC1-7): C07B59/00; C07C323/58; C12P11/00; C12P13/12

Attorney, Agent or Firm:

Michio Nakajima (2 outside)

Previous Patent: JPS5229963

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