To provide a probe holder, a probe unit, and a manufacturing method of a probe holder with excellent productivity.

This holder is formed with a conductive base member having a plurality of through-holes which penetrate into the thickness direction and install conductive probes therein respectively and a plurality of insulating holding members, formed with a kneaded material containing a thermoset resin and the resin powder, forming a hollow cylindrical shape having probe insertion through-holes through which the probes are inserted, and provided to the through-holes, respectively. The kneaded material where the mixing ratio of the resin powder is 80 volume% or less with a dielectric constant of 2.0-3.0 is more preferable.