Title:
PRODUCTION OF ENZYME PROTEIN HYDROLYSATE RICH IN DIPEPTIDE AND TRIPEPTIDE
Document Type and Number:
Japanese Patent JPS63258599
Kind Code:
A
Abstract:
Process for the enzymatic hydrolysis of proteins, employing a combination of the following three enzymes added simultaneously or successively: a) a bacterial protease which is usable in a medium in the vicinity of neutrality (pH 5 to 8), extracted from strains of Bacillus subtilis and chosen from the group comprising Neutrase< TM >, Protéase B500< TM > and H.T. Protéolytique< TM >, b) a bacterial protease which is usable in an alkaline medium (pH 7 to 11), extracted from strains of Bacillus licheniformis or Bacillus subtilis and chosen from the group comprising Alcalase< TM >, Protéase A2< TM > and Optimase< TM >, and c) a pancreatic enzyme of animal origin, including trypsin or a trypsin concentrate or the product marketed under the name PEM< TM >, under the following conditions of hydrolysis: - a neutral to alkaline pH of 7 to 10, - a temperature of 20 to 70 DEG C, and preferably 30 to 50 DEG C, - a hydrolysis period preferably not exceeding 300 minutes. Such a process enables hydrolysates rich in di- and tripeptides and having a low content of free amino acids to be obtained, for use, in particular, in artificial nutrition and in dietetics.
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Inventors:
JIYAN SHIYATOO
SERUJIYU DERUUMOO
TERANSU KAATORAITO
SERUJIYU DERUUMOO
TERANSU KAATORAITO
Application Number:
JP31730087A
Publication Date:
October 26, 1988
Filing Date:
December 15, 1987
Export Citation:
Assignee:
BELLON LABOR SA ROGER
International Classes:
A23J3/00; A23J3/34; A61K38/00; A23L1/305; A61K38/01; C12P21/06; (IPC1-7): A23L1/305; A61K37/18; C12P21/06
Attorney, Agent or Firm:
Minoru Nakamura (5 others)