PURPOSE: To obtain in high yield high-viscosity and high-purity hyaluronic acid useful as a base material for drugs and cosmetics, by chopping and making the raw material into a paste while keeping in a freezed state and sterilizing the product at high temperatures for a short time followed by quenching and then making a protease treatment.
CONSTITUTION: A raw material containing large quantities of hyaluronic acid such as cockscombs, skins or synovial fluid is rapidly freezed followed by chopping with, e.g., a meat chopper and then making the product into a paste using, e.g., a homogenizer, ultrafine grinder. This paste is then sterilized normally at 70W140°C for pref. 1secW10min followed by quenching and then performing treatment with a protease (e.g., papain, trypsin, alkalase), thus obtaining the objective hyaluronic acid. This hyaluronic acid can be prevented from quality deterioration due to hyaluronidase or prolonged heating, thus enabling improvement of its quality and yield along with significant reduction in its treatment time.
YAMADA KOJI
FUKUI FUMIO
MUTO SHINOBU
IJIJI SHUGEIZO CO LTD