複数のアンテナ構造を有する改変型糖タンパク質の生成

Japanese Patent JP5255017

B2

The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

Bobrowitz, Piotr
Hamilton, Stephen Earl.
Gangros, Tillman You.
Wilt, Stefan
Choi, Byun-Kwon
Net, Jürgen Harman
Davidson, Robert Sea.

JP2010104525A

August 07, 2013

April 28, 2010

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Glycophy, Inc.

C12N1/15; C07K14/39; C07K14/47; C12N1/18; C12N1/19; C12N9/10; C12N15/09; C12N15/12; C12N15/74; C12P21/00; C12P21/02; C12P21/06; A61K38/00; C12R1/645; C12R1/66; C12R1/72; C12R1/78

WO2001014522A1

Hiroshi Kobayashi
Eiji Katayama
Norio Omori
Yasuhito Suzuki

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