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Title:
PRODUCTION OF PEPTIDYLGLYCINE ALPHA-HYDROXYLATED MONOOXYGENASE AND USE OF THE SAME ENZYME
Document Type and Number:
Japanese Patent JPH04211367
Kind Code:
A
Abstract:

PURPOSE: To obtain peptidylglycine α-hydroxylated monooxygenase having high titer by culturing insect cells transfected with a recombinant baculovirus.

CONSTITUTION: Insect cells transfected with a recombinant baculovirus transduced with a DNA coding peptidylglycine α-hydroxylated monooxygenase is cultured. α-Hydroxyglycidyl peptide is obtained from α-hydroxylated monooxygenase obtained from the culture mixture.


Inventors:
SUZUKI KENJI
SHIMOI HIROKO
IWASAKI YASUNO
NISHIKAWA YOSHIKI
Application Number:
JP5705891A
Publication Date:
August 03, 1992
Filing Date:
March 20, 1991
Export Citation:
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Assignee:
NIHON CHIBAGAIGII KK
International Classes:
C07K1/02; C07K1/107; C07K1/113; C07K7/23; C07K14/575; C07K14/585; C07K14/60; C12N5/10; C12N9/02; C12N15/09; C12N15/10; C12N15/53; C12N15/58; C12N15/866; C12P21/02; C12Q1/26; C12R1/92; (IPC1-7): C07K1/02; C07K3/08; C07K7/36; C07K99/46; C12N5/10; C12N9/02; C12N15/10; C12N15/53; C12P21/02; C12Q1/26
Attorney, Agent or Firm:
Aoki Akira (4 outside)