PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID BY GENE RECMOBINANT BLUE-GREEN ALGA

Title:

PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID BY GENE RECMOBINANT BLUE-GREEN ALGA

Document Type and Number:

Japanese Patent JP2678209

Kind Code:

B2

Abstract:

PURPOSE: To obtain a recombinant plasmid useful for producing from microbes poly-β-hydroxybutyric acid, a biodegradable thermoplastic resin, without requiring any reduceing substance such as organocarbon source or H2.
CONSTITUTION: This recombinant plasmid pAER1 is obtained by incorporating a vector such as puc19 with a structural gene group coding enzymes composed of β-ketothiolase, acetoacetylCoA reductase and polyhydroxybutyrate polymerase each derived from Alcaligenes eutrophus. This plasmid is transfected into Synechococcus sp. to obtain transformed blue-green algae pAER1-1 (FERM P-14469), which is then cultured to accumulate poly-3b-hydroxybutyric acid in the microbes.

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Inventors:

Suzuki Taro
Masato Miyake
Yasuo Asada
Yutaka Tokiwa

Application Number:

JP31576594A

Publication Date:

November 17, 1997

Filing Date:

November 25, 1994

Export Citation:

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Assignee:

Director of Industrial Technology
Institute for Global Environmental Technology
Sekisui Chemical Co.,Ltd.

International Classes:

C12N15/09; C08L101/16; C12N1/13; C12N15/00; C12P7/62; C12R1/05; C12R1/89; (IPC1-7): C12N15/09; C12N1/13; C12P7/62

Attorney, Agent or Firm:

Toshiaki Ikeura (1 outside)
Toshiaki Ikeura

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