PURPOSE: To obtain a polysaccharide having good humectancy unaffected by humidity and film formability, by culturing a polysaccharide-productive Klebsiella oxytoca TNM3 strain or a mutant thereof and taking the product from the resultant cultured product.
CONSTITUTION: The polysaccharide-productive Klebsiella oxytoca TNM3 strain (FERM BP-4669) (or a mutant thereof), which has been found by screening microbes capable of extracellularly producing polysaccharides in high efficiency and was named so as a new microbe, is inoculated into a medium and then subjected to reciprocatingly shaking culture at 28°C for one day, and the resultant culture fluid is inoculated upon a jar fermenter and cultured under aerating agitation at 28°C for 86h while keeping the fluid at pH7, thus obtaining the objective polysaccharide made up of a main recurring unit having structure expressed by the formula and having the above advantages.
OISO YOICHI
OKUMIYA TAKESHI
SUGIHARA RYOSUKE
KAWASHIMA KAORU