PURPOSE: To obtain theanine with industrial advantage while reducing the content of caffeine or tannin by carrying out cell culture of tea using a basal medium containing ethylamine and derivatives thereof.
CONSTITUTION: A slice of stem of tea sprout is subjected to tissue culture in a basal medium containing 1-250 mM ethylamine, derivatives thereof (single substance such as ethylamine hydrogenchloride or ethylamine hydrogensulfate or mixture thereof), an ammonical nitrogen and a nitrate nitrogen in an amount larger than that of the ammoniacal nitrogen as N source under illumination at 10-32°C so as to induce callus. The resultant callus is subjected to subculture in the above-mentioned basal medium (MS-TEA medium) and to further subculture under dark. The subcultured callus is then subjected to shake culture under dark in the above mentioned basal medium, thus giving the objective theanine.
TSUNODA TAKAMI
ARAI MASAHIKO
MUKAI ISAO
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