PURPOSE: To obtain a new protein, having a specific amino acid sequence, a specified isoelectric point and a specific molecular weight, capable of efficiently carrying out the intracellular transportation of vitamin E capable of preventing phospholipids from undergoing peroxidizing reaction leading to unsaturated fatty acids and useful as medicines, etc.

CONSTITUTION: The objective protein, capable of transporting vitamin E and having partial structures of formulas I and II, 5.1 pI isoelectric point and about 30500 molecular weight is obtained by homogenizing a fresh liver of an SD rat (male) in a buffer solution, centrifuging the homogenate, collecting a supernatant, adding 1NHCl thereto, regulating the pH to 5.0, removing a precipitate, then collecting a fraction saturated with 30-60% ammonium sulfate according to an ammonium sulfate salting out method, dissolving the resultant fraction in a buffer solution, carrying out the gel filtration, collecting an active fraction having about 30000 molecular weight, passing the obtained fraction through an anion exchange column, eluting the prepared fraction with a concentration gradient at 0-0.3 M common salt concentration in the buffer solution, subsequently successively passing the fraction through a hydroxyapatite column and a gel filtration column and performing the purification.