Title:
PROTEIN COMPLEX ANALYSIS METHOD
Document Type and Number:
Japanese Patent JP3947775
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method of determining the interaction interface of protein adjoining other molecules in a complex containing protein with high sensitivity and a high efficiency comparing to conventional methods.
SOLUTION: By substituting deuterium for hydrogen except arbitrary hydrogens (referred to as 'specific hydrogen', below) existing in the side-chains of a protein as one molecule among a plurality of molecules comprising a complex, the position of specific hydrogens existing within 10 &angst from the hydrogens contained in other molecules adjoining the protein is determined based on a cross saturation phenomena using NMR method. As the result, the method enables to determine the interaction interface of the complex containing the protein with high sensitivity and a high efficiency comparing to conventional methods.
Inventors:
Takao Hashio
Kazuo Shimada
Satoshi Nakamura
Kazuo Shimada
Satoshi Nakamura
Application Number:
JP2002217938A
Publication Date:
July 25, 2007
Filing Date:
July 26, 2002
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
AJINOMOTO CO.,LTD.
AJINOMOTO CO.,LTD.
International Classes:
G01R33/465; C12Q1/68; G01N24/08; (IPC1-7): G01R33/465; C12Q1/68; G01N24/08
Domestic Patent References:
| JP2002031610A |
Other References:
Rosen et al.,Selective methyl group protonation of perdeuterated proteins,Journal of Molecular Biology,NL,Elsevier;Amsterdam,1996年,263/5,627-636
Goto et al.,A robust and cost-effective method for the production of Val, Leu, Ile(δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins ,Journal of Biomolecular NMR,NL,Kluwer Academic Publ.;Dordrecht,1999年 1月15日,Vol. 13,369 - 374
Goto et al.,A robust and cost-effective method for the production of Val, Leu, Ile(δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins ,Journal of Biomolecular NMR,NL,Kluwer Academic Publ.;Dordrecht,1999年 1月15日,Vol. 13,369 - 374
Attorney, Agent or Firm:
Yasumasa Ishida
Kato Asamichi
Kato Asamichi
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