Title:
PURIFICATION OF CHOLESTEROL OXIDASE
Document Type and Number:
Japanese Patent JPS5878586
Kind Code:
A
Abstract:
A method for the purification of cholesterol oxidase is disclosed. The method comprises the steps of:(a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition so as to adsorb the cholesterol oxidase on the composition;(b) separating the solution from the chromatography composition; and(c) contacting the chromatography composition with an eluting solution containing a surfactant so as to recover the cholesterol oxidase;characterized in that(1) the chromatography composition comprises an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase and(2) the concentration of the surfactant in the eluting solution is between 0.0001 and 10 weight percent of the eluting solution.
Inventors:
ROI YUUJIIN SUNOOKU
CHIYAARUZU TOOMASU GUTSUDOHIYU
CHIYAARUZU TOOMASU GUTSUDOHIYU
Application Number:
JP18225682A
Publication Date:
May 12, 1983
Filing Date:
October 19, 1982
Export Citation:
Assignee:
EASTMAN KODAK CO
International Classes:
C12N9/04; (IPC1-7): C12N9/04
Domestic Patent References:
| JPS5476893A | 1979-06-19 | |||
| JPS5437885A | 1979-03-20 |
Attorney, Agent or Firm:
Aoki Akira
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