Title:
精製方法
Document Type and Number:
Japanese Patent JP4831697
Kind Code:
B2
Abstract:
The present invention relates to a method of isolating a target compound from other components of a liquid, which method comprises at least two chromatographic steps, in any sequence of order, wherein the mobile phase is contacted with an affinity chromatography matrix and/or an ion-exchange chromatography matrix and/or a hydrophobic interaction chromatography matrix, wherein the contacting with at least one of the matrices takes place in the presence of at least one non-ionic polyether; and obtaining the target compound(s) in a separate fraction from the last chromatographic step. In the most preferred embodiment, the non-ionic polyether is poly(ethylene glycol) (PEG).
Inventors:
Van alstin, james
Hamushmand, Hamid
Lunglov, Anders
Oberg, Par-Michael
Hamushmand, Hamid
Lunglov, Anders
Oberg, Par-Michael
Application Number:
JP2007506111A
Publication Date:
December 07, 2011
Filing Date:
March 31, 2005
Export Citation:
Assignee:
GE Healthcare Bioscience Activolag
International Classes:
G01N30/26; B01D15/08; B01D15/32; B01D15/36; B01D15/38; C07K1/22; C07K1/36; G01N30/00; G01N30/02; G01N30/46; G01N30/88; B01D
Domestic Patent References:
| JP5500923A |
Foreign References:
| WO2002036245A1 | 2002-05-10 | |||
| US20040030107A1 | 2004-02-12 |
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Toshihisa Kurokawa
Hirokazu Ogura
Toshihisa Kurokawa