PURPOSE: To purify a reaction liquid prepared by the use of the titled microbial cell or enzyme as a catalyst in an aqueous medium, stably for a long period, by combining a filtration process using a porous hollow fiber membrane made of polyethylene and having a specific bubble point with a process comprising of washing under specific condition.
CONSTITUTION: Fine solid particles are removed from a reaction liquid obtained by using microbial cells, immobilizd microbial cells or immobilized enzymes as a catalyst in an aqueous medium, especially from an aqueous solution of acrylamide. The removal is carried out by repeating (A) the filtration of the solution with a porous hollow fiber membrane made of a polyethylene and having a bubble point of 1W20kg/cm2 and (B) the washing of the clogged hollow fiber membrane, especially by a combination of said hollow fiber membrane is made to ≥3kg/cm2 before washing to recover the filtering performance of the hollow fiber membrane completely and to purify the above reaction liquid economically. The consumption of chemicals such as alkali, etc., can be decreased, and the amount of waste water to be treated is also decreased by this process.
YAMAGUCHI YASUMASA
NISHIDA MASASHI
DOI TOSHIAKI
