Title:
組換え産生ポリペプチドの精製
Document Type and Number:
Japanese Patent JP6903092
Kind Code:
B2
Abstract:
Described herein is a method for separating a recombinantly produced polypeptide from host cell protein. The method includes a step of loading a clarified cell culture supernatant that includes the recombinantly produced polypeptide and the HCP onto a Protein A chromatography column and washing the Protein A chromatography column with a wash buffer comprising a fatty acid having a chain length of at least about 6 carbon atoms, or a fatty acid salt thereof to remove HCP and then recovering the recombinantly produced polypeptide.
Inventors:
Gruber, David, Edwin
Turner, richard, edward
Bee, Jared, Samuel
Ahdar, Christopher, Douglas
Tea, Riu
Turner, richard, edward
Bee, Jared, Samuel
Ahdar, Christopher, Douglas
Tea, Riu
Application Number:
JP2019075866A
Publication Date:
July 14, 2021
Filing Date:
April 11, 2019
Export Citation:
Assignee:
Medimun Limited
Mediemu, LRC
Mediemu, LRC
International Classes:
C07K1/22; C07K16/24; C12P21/08
Domestic Patent References:
| JP2009537608A | ||||
| JP2011524750A | ||||
| JP2012506385A | ||||
| JP2012506383A | ||||
| JP2013508387A |
Foreign References:
| WO2011073389A1 | ||||
| WO2012020038A1 |
Other References:
Journal of Chromatography B (2005) Vol.814, pp.209-215
ELIN MONIE,Evaluation of the 96-well format for screening of chromatographic buffer conditions,Master's degree project,Uppsala University,2006年10月,UPTEC X 06 020 [online],第16-17頁, 第24頁, 第51-53頁,ISSN:1401-2138, 2018年4月6日検索,URL,
Bioengineered (2013) Vol.4, No.5, pp.288-291 (published online 2013 Jan 17)
Journal of Chromatography A (2005) Vol.1079, pp.372-381
ELIN MONIE,Evaluation of the 96-well format for screening of chromatographic buffer conditions,Master's degree project,Uppsala University,2006年10月,UPTEC X 06 020 [online],第16-17頁, 第24頁, 第51-53頁,ISSN:1401-2138, 2018年4月6日検索,URL,
Bioengineered (2013) Vol.4, No.5, pp.288-291 (published online 2013 Jan 17)
Journal of Chromatography A (2005) Vol.1079, pp.372-381
Attorney, Agent or Firm:
Hiraki International Patent Office