PURPOSE: To quantitatively determine γ-polyglutamic acid (γ-PGA) which is the base of the quality standard of fermented soybeans and being developed as a calcium myenteric absorbing agent simply, quickly, and easily, and also measure the molecular weight.
CONSTITUTION: After a sample containing γ-PGA is processed in polyacrylamidogel by electrophoresis without performing particular treatment, a gel flat plate or a gel disc is at first colored with Coomassie brilliant blue dye, further colored with basic dye such as methylene blue or safranine-O after decoloring, further after decoloring densitometry is performed, and hence γ-PGA can be quantatively determined. In this method, molecular weight marker is also subjected to electrophoresis simultaneously, and hence the molecular weight can be measured.
OGAWA YOSHIHIRO
YAMAZAKI EMIKO
YUASA KATSUMI
MOTAI HIROSHI
