Title:
電気パルスを用いたRNAのトランスフェクション方法
Document Type and Number:
Japanese Patent JP2004528038
Kind Code:
A
Abstract:
The present invention relates to a method of inserting RNA into cells. In this method, cells are transfected with RNA using electroporation in order to achieve high transfection efficiency. The method is useful, inter alia, in providing cells to be used in cell-based therapies, e.g. in preparing cells useful as anti-cancer vaccines. Preferably, the RNA has a 5' cap and a 3' poly (A) tail.
Inventors:
Gaudernack, Gustav
Sabyoe-Lassen, Stein
Sabyoe-Lassen, Stein
Application Number:
JP2002587615A
Publication Date:
September 16, 2004
Filing Date:
April 22, 2002
Export Citation:
Assignee:
GEMVAX AS
International Classes:
C12N15/09; A61K31/7105; A61K39/00; A61K48/00; A61P35/00; C12N15/87; (IPC1-7): C12N15/09; A61K31/7105; A61K39/00; A61K48/00; A61P35/00
Attorney, Agent or Firm:
Michiteru Soga
Michiharu Soga
Hidetoshi Furukawa
Suzuki Kenchi
Kajinami order
Michiko Daigo
Michiharu Soga
Hidetoshi Furukawa
Suzuki Kenchi
Kajinami order
Michiko Daigo