Title:
REACTION OF NUCLEIC ACID HYBRIDIZATION UTILIZING ULTRASONIC WAVE AND APPARATUS THEREFOR
Document Type and Number:
Japanese Patent JP3959652
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To improve the efficiency of the subject reaction such as clinical tests, food tests or environmental tests by reacting an RNA or a DNA with a nucleic acid having a sequence complementary thereto while applying ultrasonic waves with a modulated amplitude thereto.
SOLUTION: An RNA or a DNA is reacted with a nucleic acid having a sequence complementary thereto while applying ultrasonic waves which are pulsating and amplitude modulated ones, having a modulated amplitude at 20-40kHz and generated according to an amplitude modulating method for pulsatingly changing the applied voltage of an ultrasonic vibrator with time at 50 or 60 cycles/sec thereto with 1-10W electric power for 2-10min and the produced reactional product is then measured to thereby improve the efficiency in a nucleic acid hybridization reaction utilizable within a wide range of industrial fields such as clinical tests, food tests and environmental tests and detect the nucleic acid in a sample.
Inventors:
Yoichi Monjo
Bunsei Kawakami
Yoshihisa Kawamura
Bunsei Kawakami
Yoshihisa Kawamura
Application Number:
JP26180096A
Publication Date:
August 15, 2007
Filing Date:
October 02, 1996
Export Citation:
Assignee:
TOYO BOSEKI KABUSHIKI KAISHA
International Classes:
C12Q1/68; G01N29/00; C07H21/00; C12M1/42; C12N13/00; C12N15/09; (IPC1-7): C12Q1/68; C07H21/00; C12M1/42; C12N13/00; C12N15/09; G01N29/00
Domestic Patent References:
| JP6054315B2 | ||||
| JP2043000A |
Other References:
Flavell, R.A., et al.,Eur. J. Biochem.,1974年,Vol.47(3),p.535-43