Title:
REACTIVE SOLID-PHASE CARRIER AND DNA FRAGMENT DETECTING TOOL
Document Type and Number:
Japanese Patent JP3857075
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a fixing method by which a pre-prepared DNA fragment, a nucleotide derivative such as the oligonucleotide, etc., or its analogue can be fixed densely and stably to the surface of a solid-state carrier through a quick reaction, a method of manufacturing a carrier for fixing the nucleotide derivative or its analogue utilizing the fixing method, and a detecting tool for detecting nuclei acid fragment samples, etc., represented by a DNA chip.
SOLUTION: A reactive solid-phase carrier is constituted by fixing a group of vinylsulfonyl radicals or their reactive precursor radicals to the rugged surface of the solid-phase carrier by using a divinylsulfon compound, etc., by performing covalent coupling through their coupling radicals. The nuclei acid fragment sample detecting and fixing tool is constituted by coupling and fixing the nucleotide derivative, such as the oligonucleotide, polynucleotide, peptide nuclei acid, etc., or its analogue to the reactive solid-phase carrier by performing covalent coupling.
Inventors:
Hiroshi Shinoki
Osamu Seshimoto
Osamu Seshimoto
Application Number:
JP2001168982A
Publication Date:
December 13, 2006
Filing Date:
June 05, 2001
Export Citation:
Assignee:
FUJIFILM Holdings Co., Ltd.
International Classes:
G01N33/53; C12M1/00; C12N15/09; C12Q1/68; G01N33/547; G01N33/552; G01N33/553; G01N37/00; (IPC1-7): G01N33/53; C12M1/00; C12N15/09; C12Q1/68; G01N33/547; G01N37/00; //G01N33/552; G01N33/553
Domestic Patent References:
| JP200113103A | ||||
| JP8233773A |
Foreign References:
| WO2000061282A1 | ||||
| WO2000004382A1 |
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes
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