RECOMBINANT ANTIGEN FROM NS-3 REGION OF HEPATITIS C VIRUS

Japanese Patent JPH08127592

A

To obtain the subject new polypeptide useful as an antigen used for an immunological test for hepatitis C virus or a helicase protein, consisting of a region of non-structural protein of the hepatitis C virus and having a foreign amino acid sequence.

This polypeptide has an amino acid sequence containing an amino acid sequence expressed by the formula, derived from a non structural protein 3 (NS-3) region of hepatitis C virus, consisting of (1207±10)-(1488±10) amino acid sequence of the hepatitis C virus, containing ≤20 foreign amino acids, and useful as an antigen used in an immunological test of the hepatitis C or as a helicase protein. The polypeptide is obtained by using an NS3 clone containing a gene information from the NS-3 region, cloning the NS-3 by using a pair of primers consisting of a part of a base sequence thereof in a PCR method, incorporating the obtained DNA into an expression vector, and expressing the polypeptide in a host cell.

KURISUTOFU SAIDERU
URUSURAAHENRIKE BUIINHIYUSU
URUBAN SHIYUMITSUTO
MANFURETSUDO MOTSUTSU
MITSUSHIERU BUIIDOMAN
BAABARA ATSUPUMAIAA
ERUBUIN SOUTOSHIETSUKU

JP20708695A

May 21, 1996

August 14, 1995

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BOEHRINGER MANNHEIM GMBH

G01N33/53; A61K39/29; A61P1/16; A61P31/12; A61P31/14; C07H21/04; C07K14/02; C07K14/10; C07K14/18; C07K16/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/40; C12N15/51; C12P21/02; C12P21/04; G01N33/532; G01N33/537; G01N33/569; G01N33/576; G01N33/68; A61K38/00; C12R1/19; (IPC1-7): C07K14/18; C07H21/04; C12N1/21; C12N15/09; C12P21/02; G01N33/53; G01N33/576

JPH06505164A	1994-06-16
JPH05508219A	1993-11-18

Yusuke Hiraki (2 outside)

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