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Title:
RECOMBINANT DNA AND ITS USE
Document Type and Number:
Japanese Patent JPS61111690
Kind Code:
A
Abstract:

PURPOSE: To prepare a human Cu, Zn-superoxide dismutase gene in high efficiency, by synthesizing a recombinant DNA having a human Cu, Zn-superoxide dismutase structure gene at the downstream of a plasma expression controlling gene.

CONSTITUTION: A microorganism transformed with a recombinant DNA having human Cu, Zn-superoxide dismutase structure gene at the downstream of a plasma expression controlling gene, is cultured, and the human Cu, Zn-superoxide dismutase accumulated in the culture product is separated therefrom. In the case of using Escherichia coli, the cultivation is carried out e.g. in an L medium, an M9 medium containing glucose and casamino acid, etc. usually at about 15W43°C for about 2W24hr. An induced synthesis can be performed by adding a drug such as mitomycin C to the culture product or irradiating the culture product with ultraviolet radiation in logarithmic growth stage.


Inventors:
KUMAHARA HIROMI
MARUYAMA MASAO
AHEIJI SHIGEHARU
OWA TAKUJI
Application Number:
JP23239584A
Publication Date:
May 29, 1986
Filing Date:
November 06, 1984
Export Citation:
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Assignee:
UBE INDUSTRIES
International Classes:
C12N15/09; C07H21/04; C12N1/20; C12N9/02; C12N15/70; C12R1/19; (IPC1-7): C07H21/04; C12N1/20; C12N9/02; C12N15/00; C12R1/19
Domestic Patent References:
JPS60137286A1985-07-20
Attorney, Agent or Firm:
Heiyoshi Odashima



 
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