RECOMBINANT DNA MOLECULE ENCODING TUMOR NECROSIS FACTOR

Japanese Patent JP2548525

B2

PURPOSE: To obtain the constituting molecule containing a DNA encoding a tumor necrosis factor(TNF), having improved cytotoxicity and cytostatic activity against tumor cells, capable of mass producing TNF-like polypeptide such as antitumor agent, etc., in a prokaryotic cell.
CONSTITUTION: A recombinant DNA molecule selected from the group consisting of a DNA insert (DSM 3, 159) of p-mouse tumor necrosis factor (mTNF)-3, a DNA insert (DSM 3, 160) of p-human tumor necrosis factor (hTNF) 1, a DNA sequence hybridizing either or both of these DNA inserts and encoding a polypeptide showing biological activity in expression and a DNA sequence foregoing either of the DNA insert or the DNA sequence, acting on and bonded to an expression control sequence such as T4, pL-T4 or the like is expressed in a prokaryotic host and the prepared TNF-like polypeptide-containing composition is brought into contact with an anion exchanger and the adsorbed material is eluted to give the TNF-like polypeptide having antitumor activity in a high yield.

FUAIAAZU UORUTAA SHII
FURANSEN RUSHIA EMU
TABERUNIA JAN HONOORU REO
MARUMENAUTO ANII RUISU MARII
BAN DERU HEIDEN JOZE
ARETSUTO BERUNAARU
KAWASHIMA ERITSUKU EICHI

JP2627195A

October 30, 1996

January 19, 1995

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BAIOJEN INC

A61K38/00; A61K38/19; A61K38/21; A61K45/00; A61P33/02; G01N33/577; A61P35/00; C07H21/00; C07H21/04; C07K1/18; C07K14/00; C07K14/52; C07K14/525; C07K14/555; C07K14/56; C07K14/565; C07K14/57; C12N15/00; C12N15/09; C12N15/28; C12N15/70; C12P21/00; C12P21/02; C12Q1/02; C12R1/19; A61K; C07H; C07K; C12N; C12P; (IPC1-7): C12N15/09

Haruo Hamada

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