PURPOSE: To produce a large amount of human prolactin (HP) by transducing DNA integrated with a promoter, Shine-Dalgano sequence and translation initiation codon at the upper stream of gene to code HP into Escherichia coli.
CONSTITUTION: A promoter (preferably tryptophan base, lactose base, tack base, Pierre base, etc.), Shine-Dalgano sequence (SD sequence) and translation initiation codon are integrated in this order to the upper stream of a gene to code HP polypeptide to give recombinant DNA for producing HP. In the operation, the promoter has 16-18 pairs of base pairs existing between positions -10 to -35 from the transfer initiation point of mRNA and the SD sequence and the translation initiation codon. The recombination, for example, is carried out by making pL100 from plasmid phPRL9 and PUC19. The recombinant DNA is transduced into Escherichia coli, which is transformed and the bacterium is cultivated to produce HP.
ICHIKAWA TATSUO
NAKAJIMA KUNIO
| JPS61202690A | 1986-09-08 |