PURPOSE: To prepare prolactin of mouse by transforming a bacterium with a recombinant DNA for producing prolactin of mouse, culturing the transformant and separating polypeptide of murine prolactin from the culture mixture.
CONSTITUTION: The upper stream of a gene coding polypeptide of murine prolactin is integrated with a promoter, Shine-Dalgarno sequence and the translation ignition codon in this order to prepare a recombinant DNA for producing murine prolactin. Then, a bacterium is transformed using the recombinant DNA, the transformed bacterium is cultured and polypeptide of murine prolactin (mPRL polypeptide) is separated from the culture mixture. When this method is applied, a large amount of mPRL polypeptide is produced and the method is useful for studying the treatment of various diseases.
HARIGAI TOSHIO
NAKAJIMA KUNIO
| JPS61170392A | 1986-08-01 |