Title:
RECOMBINANT DNA STRUCTURE ENCODING NUCLEOSIDE DIPHOSPHATE KINASE AND ITS PRODUCT
Document Type and Number:
Japanese Patent JPH04271786
Kind Code:
A
Abstract:
PURPOSE: To provide a recombinant DNA structure encoding the gene of a nucleoside diphosphate kinase, and to provide a method for producing an excessive amount of the enzyme in E. coli.
CONSTITUTION: A polypeptide having a NDP kinase activity is disclosed. A recombinant DNA structure encoding a gene related to the nucleoside diphosphate kinase is also disclosed. A transformed host cell containing the structure is also disclosed. An isolated DNA sequence of a prokaryotic cell nucleotide diphosphate kinase gene encoding the protein having a sub-unit mol.wt. of about 16,000 dalton and lacking a cysteine residue is also disclosed.
Inventors:
MASAYORI INOUE
HOSE MUNOSUUDORADO
SUMIKO INOUE
HOSE MUNOSUUDORADO
SUMIKO INOUE
Application Number:
JP41597490A
Publication Date:
September 28, 1992
Filing Date:
December 28, 1990
Export Citation:
Assignee:
UNI MEDEISHIN ANDO DENTEISUTOR
International Classes:
C07K14/195; C07K14/00; C07K14/41; C12N1/21; C12N9/12; C12N15/09; C12N15/54; C12R1/01; C12R1/19; (IPC1-7): C07K13/00; C12N1/21; C12N9/12; C12N15/54
Attorney, Agent or Firm:
Kazuo Sato (2 outside)
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