Title:
RECOMBINANT RICIN TOXIN FRAGMENT
Document Type and Number:
Japanese Patent JP2574142
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new toxin fragment having a specific amino acid sequence, not containing castor bean cell, obtained by recombinant genetic engineering, having cytotoxicity and used for medicines or the like for killing target cell such as tumor cell.
SOLUTION: This new recombinant ricin B fragment comprises a recombinant ricin B not containing castor bean cell substance and is subunit of ricin having cytotoxicity and produced by Escherichia coli or yeast cell transformed with DNA encoding an amino acid sequence represented by the formula, and the ricin B subunit has action attaching A subunit having cytotoxicity to a target cell to be killed and promoting transfer of A subunit to the interior of cytoplasm. The ricin B is obtained by screening cDNA library prepared according to ordinary method by using mRNA extracted from seed of Ricinus communis L. and expressing DNA encoding the resultant ricin B with Escherichia coli or yeast cell.
Inventors:
GUREN TOOMASU HOON
MAIKERU PIATATSUKU JUNIA
MAIKERU PIATATSUKU JUNIA
Application Number:
JP20122795A
Publication Date:
January 22, 1997
Filing Date:
August 07, 1995
Export Citation:
Assignee:
SHIITASU ONKOROJII CORP
International Classes:
A61K38/36; A61K35/12; A61K35/74; A61K36/06; A61K36/47; A61K38/00; A61K39/44; C07K14/415; C07K14/42; C12N1/19; C12N1/21; C12N15/09; C12N15/10; C12N15/69; C12N15/70; C12P21/02; C12R1/19; C12R1/865; (IPC1-7): C07K14/42; C12P21/02
Domestic Patent References:
| JP60102188A | ||||
| JP61108388A |
Attorney, Agent or Firm:
Shusaku Yamamoto