Title:
REDUCTION OF NONSPECIFIC HYBRIDIZATION BY USING NOVEL BASE-PAIRING SCHEMES
Document Type and Number:
Japanese Patent JP2008048741
Kind Code:
A
Abstract:
To provide a nucleic acid hybridization assay which can reduce background noises to increase accuracy for detecting and determining analytes.
The nucleic acid hybridization assay is for detecting nucleic acid analytes in a sample with a plurality of assay components. Each of the assay components contains at least one hybridizable oligonucleotide segment, and the nucleic acid hybridization assay is improved to include a process for taking the first nucleotide unit not effectively forming a base pair with adenosine (A), thymidine (T), cytidine (C), guanosine (G), or uridine (U) under a condition for forming A-T and G-C base pairs in at least one hybridizable oligonucleotide segment.
Inventors:
COLLINS MARK L
HORN THOMAS
SHERIDAN PATRICK E
WARNER BRIAN D
URDEA MICHAEL S
HORN THOMAS
SHERIDAN PATRICK E
WARNER BRIAN D
URDEA MICHAEL S
Application Number:
JP2007273226A
Publication Date:
March 06, 2008
Filing Date:
October 19, 2007
Export Citation:
Assignee:
BAYER CORP
International Classes:
C12N15/09; C07H19/16; C12Q1/68; C07H19/20; C07H21/00; C12N15/113; C12Q1/6811; C12Q1/6813; C12Q1/682; C12Q1/6832; C12Q1/6837
Domestic Patent References:
| JPS62188970A | 1987-08-18 |
Attorney, Agent or Firm:
Hidesaku Yamamoto