PURPOSE: To regenerate old SAKE into fresh SAKE having fresh fragrance by successively passing SAKE to be refined, having change of quality with time through an immobilized yeast tank and an immobilized AAT (alcohol acetyl transferase active yeast) tank.

CONSTITUTION: SAKE to be refined, having the above-mentioned change of quality with time is passed through an immobilized yeast tank, which is prepared by culturing a specific yeast (e.g. malic acid producing strong yeast [NYUBO]) for regenerating old SAKE and by immobilizing with an inert support material, as a primary treatment. Then the SAKE having finished the primary treatment is passed through an immobilized AAT tank obtained by immobilizing AAT having properties of forming GINSANKO which abnormally metabolizes SAKE to an insoluble support material in order to carry out a secondary treatment. Consequently, SAKE to be treated is purely zymologically regenerated to give fresh SAKE.