Title:
CYP1A2の誘導を評価するレポーターベクター
Document Type and Number:
Japanese Patent JP4986061
Kind Code:
B2
Abstract:
A reporter vector which can evaluate the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 and a method for evaluation of the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 by using the reporter vector. A reporter system which can evaluate the ability of a drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 is completed by constructing a reporter vector having a reporter gene linked to the 3' end of a region between CYP1A1 and CYP1A2 or a reporter vector having different reporter genes linked to the both ends of the region, respectively, so as to sandwich the region, and a reporter vector having a deletion mutation in the region, and confirming that the expression of a reporter molecule is increased by the drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 in the reporter system using the reporter vector.
Inventors:
Ueda Rika
Kusano Kazutomi
Yamazoe Yasushi
Kiyoshi Nagata
Kusano Kazutomi
Yamazoe Yasushi
Kiyoshi Nagata
Application Number:
JP2007524690A
Publication Date:
July 25, 2012
Filing Date:
July 12, 2006
Export Citation:
Assignee:
Eisai R & D Management Co., Ltd.
Tohoku University
Tohoku University
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12Q1/02; G01N33/15; G01N33/50
Other References:
CORCHERO, J. et al.,Organization of the CYP1A cluster on human chromosome 15: implications for gene regulation.,Pharmacogenetics,2001年,Vol. 11, No. 1,p. 1-6
QUATTROCHI, L. C. et al.,The human CYP1A2 gene and induction by 3-methylcholanthrene. A region of DNA that supports AH-receptor binding and promoter-specific induction., J. Biol. Chem.,1994年,Vol. 269, No. 9,p. 6949-6954
POSTLIND, H. et al.,Response of human CYP1-luciferase plasmids to 2,3,7,8-tetrachlorodibenzo-p-dioxin and polycyclic aromatic hydrocarbons.,Toxicol. Appl. Pharmacol.,Vol. 118, No. 2,p. 255-262
QUATTROCHI, L. C. et al.,The human CYP1A2 gene and induction by 3-methylcholanthrene. A region of DNA that supports AH-receptor binding and promoter-specific induction., J. Biol. Chem.,1994年,Vol. 269, No. 9,p. 6949-6954
POSTLIND, H. et al.,Response of human CYP1-luciferase plasmids to 2,3,7,8-tetrachlorodibenzo-p-dioxin and polycyclic aromatic hydrocarbons.,Toxicol. Appl. Pharmacol.,Vol. 118, No. 2,p. 255-262
Attorney, Agent or Firm:
Yoshiyuki Kawaguchi
Hidemi Matsukura
Tsutomu Toyama
Hidemi Matsukura
Tsutomu Toyama