Title:
リボ核酸固定化担体及びそれを用いたリボ核酸の分離回収方法
Document Type and Number:
Japanese Patent JP6667896
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide novel carriers for ribonucleic acid separation and purification as well as uses thereof, by overcoming the problems that conventional nucleic acid separation carriers could not solve, such as uses of nucleic acid denaturing reagents including chaotropic agents, heat resistance, organic solvent resistance, and mechanical strength of carriers, regeneration by calcination of carriers, and high cost surface ligands such as oligo dT.SOLUTION: Aminated mesoporous silica particles which adsorb and separate ribonucleic acid in a large amount are obtained by controlling the amount of amino groups contained in aminated mesoporous silica particles having mesopores capable of selectively adsorbing ribonucleic acid, particularly silica particles, the pore diameters of the silica particles, and their particle sizes.SELECTED DRAWING: Figure 1
Inventors:
Katsuya Kato
Mr. Nagata Hisae
Mr. Nagata Hisae
Application Number:
JP2015218331A
Publication Date:
March 18, 2020
Filing Date:
November 06, 2015
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
International Classes:
C12N15/10; B01D15/00; B01J20/22; B01J20/30; C12Q1/6806
Domestic Patent References:
| JP2008116265A | ||||
| JP2003321219A | ||||
| JP2005333936A | ||||
| JP2014532071A | ||||
| JP2013036865A |
Other References:
Steinbacher J. L. et al., "Adsorption and release of siRNA from porous silica.", Langmuir, 2014 Apr 22, vol,. 30, no. 15, pp. 4396-4405
Attorney, Agent or Firm:
Yasuko Eto
Satoru Matsumoto
Masaki Okui
Junko Yoshimizu
Satoru Matsumoto
Masaki Okui
Junko Yoshimizu