ROLLING CIRCLE REPLICATION OF PADLOCK PROBE

Title:

ROLLING CIRCLE REPLICATION OF PADLOCK PROBE

Document Type and Number:

Japanese Patent JP2009240312

Kind Code:

A

Abstract:

To solve the problem that various methods are in use for detecting single or multiple nucleotide variations, and most methods depend on amplification of a target sequence prior to analysis, commonly by PCR, so that valuable information on the localization of allelic variants is lost.

A method includes cutting a target nucleic acid near or preferably at the site which hybridizes with the padlock probe, whereby the 3'-end of the cut target nucleic acid acts as a primer for rolling circle replication of the padlock probe. Also included is a method of assaying a polyepitopic target by the use of two affinity probes each carrying an oligonucleotide tag and of a padlock probe for rolling circle replication in association with the two affinity probes.

Inventors:

LANDEGREN ULF

Application Number:

JP2009108689A

Publication Date:

October 22, 2009

Filing Date:

April 28, 2009

Export Citation:

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Assignee:

LANDEGREN ULF

International Classes:

C12N15/09; C12Q1/6816; C12Q1/68; C12Q1/6844; G01N33/53

Foreign References:

WO1997019193A2	1997-05-29
WO1998004745A1	1998-02-05
WO1998004746A1	1998-02-05

Attorney, Agent or Firm:

Takehiko Suzue
Toshio Shirane
Sadao Muramatsu

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