Title:
SCREENING METHOD OF ANTIBIOTIC PRODUCTION-RELATED GENE
Document Type and Number:
Japanese Patent JP2015167482
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a screening method of an antibiotic production-related gene which enables exploration of antibiotics produced by soil microorganisms and the like which are difficult to culture.SOLUTION: The method comprises adding a substance for activating the production of an antibiotic by microorganisms into an environmental sample in which microorganisms inhabit; subsequently extracting RNA from the environmental sample; and selecting an antibiotic production-related gene-derived mRNA comprised in the extracted RNA by meta-transcriptome analysis. According to the invention, besides microorganisms which is difficult to culture, exploration of antibiotics produced by microorganisms which are not known until now can be achieved.
Inventors:
FUJII TAKESHI
NAZARI BEHNAM
NAZARI BEHNAM
Application Number:
JP2014042420A
Publication Date:
September 28, 2015
Filing Date:
March 05, 2014
Export Citation:
Assignee:
NAT INST AGRO ENVIRONMENTAL SCIENCE
International Classes:
C12Q1/68; C12N15/09; C12P1/02; C12P1/04; C12P1/06
Other References:
農業環境技術研究所研究成果情報(2013), VOL.29, P.38-39, JPN6018001035
APPL. MICROBIOL. BIOTECHNOL.(2012), VOL.96, P.793-802, JPN6018001034
APPL. MICROBIOL. BIOTECHNOL.(2012), VOL.96, P.793-802, JPN6018001034
Attorney, Agent or Firm:
Yuji Tsujida
Yoshihiro Shimizu
Shinichi Abe
Yoshihiro Shimizu
Shinichi Abe
Previous Patent: FLUID SUPPLYING NOZZLE
Next Patent: FOAMABLE OIL-IN-WATER TYPE EMULSIFIED FAT COMPOSITION
Next Patent: FOAMABLE OIL-IN-WATER TYPE EMULSIFIED FAT COMPOSITION