Title:
破骨細胞形成抑制因子の産生を調節し得る物質のスクリーニング方法
Document Type and Number:
Japanese Patent JP4336467
Kind Code:
B2
Abstract:
The present invention has the object to provide an agent for controlling the production of osteoprotegerin (OPG) and a method for screening substances capable of controlling the production of OPG, and it solves the object by providing the agent for controlling the production of OPG, which comprises a saccharide as an effective ingredient; and a method for screening a substance capable of controlling the production of OPG, which comprises the steps of culturing an established cell line capable of producing OPG in a nutrient culture medium; adding a test sample to the cell culture; culturing the cells in the presence of the test sample to produce OPG; assaying the production level of OPG in the culture supernatant; and judging the test sample to be a positive substance that can control the production of OPG when the production level of OPG for the test sample is higher or lower than that for a control system.
Inventors:
Toshio Ariyasu
Toshiharu Hanatani
Arai Shigeyuki
Masao Ikeda
Masashi Kurimoto
Toshiharu Hanatani
Arai Shigeyuki
Masao Ikeda
Masashi Kurimoto
Application Number:
JP2001317358A
Publication Date:
September 30, 2009
Filing Date:
October 15, 2001
Export Citation:
Assignee:
Hayashibara Institute of Biochemistry
International Classes:
C12Q1/02; G01N33/50; A61K31/70; A61K31/7004; A61K31/7016; A61K33/06; A61K45/06; A61P19/00; A61P19/02; A61P19/08; A61P43/00; C07H3/02; C07H3/04; G01N33/15
Domestic Patent References:
| JP2000198736A | ||||
| JP2001122798A | ||||
| JP2001231573A |
Other References:
Cell, Volume 89, Issue 2, 18 April 1997, Pages 309-319
Biochemical and Biophysical Research Communications, 1998, 248(3),p696-700
Biochemical and Biophysical Research Communications, 1998, 248(3),p696-700