PURPOSE: To separate and determine catabolic hemoglobin in high accuracy, by using a copolymer constituting of a specific rate of three kinds monomers, each having the hydrophobic parameter lower than the specific value, of a monomer having COOH radical in a molecule less than a specific value, a cross-linking polymeric monomer and a monomer not containing COOH as packings.
CONSTITUTION: The fine particles of a copolymer copolymerized three kinds of monomers, each having ≤2.3 hydrophobic parameter are used for packings of liquid chromatography. That is, copolymer particles constituted by 5W95wt% monomer A having ≥one COOH radical and one polymeric double bond, 10W 95wt% cross-linking monomer B having ≥2 polymeric double bond and 0W 85wt% monomer C which is other than the group A and having one polymeric double bond are obtained by crushing after said copolymer is obtained by a suspension polymerization method or bulk polymerization method. Catabolic hemoglobin in blood serum of a diabetic etc. is separated and is determined in a short time in high accuracy by using this particle as packings.
OBARA MINORU
NAGATA MITSUO
ARAKI YASUHIKO
| US4139684A | 1979-02-13 | |||
| US4238196A | 1980-12-09 |