SEPARATION OF PEPTIDE ANTIBIOTICS K582M-A AND K582M-B

Title:

SEPARATION OF PEPTIDE ANTIBIOTICS K582M-A AND K582M-B

Document Type and Number:

Japanese Patent JPS59198990

Kind Code:

A

Abstract:

PURPOSE: To separate peptide antibiotics K582M-A and K582M-B from their mixture, in high efficiency, by adsorbing the mixture with a porous polymer resin free of ion exchange group, and eluting the antibiotics separately with water having different pH.

CONSTITUTION: An aqueous solution containing a mixture of peptide antibiotics K582M-A and K582M-B and obtained by the cultivation of metarhizium anisopliae (Metsch) Sorok. var. anisopliae 582 strain (FERM-P No.4217), is adjusted to about 9.5pH, and is made to contact with 300W500ml (based on 1g of the mixture) of a porous polymer resin free of ion exchange group to effect the adsorption of the mixture. The resin is treated with water of ≥5pH to collect the fraction containing K582M-B as main component, and the residue is treated with water of ≤5pH to collect the fraction containing K582M-A as main component.

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Inventors:

MAJIMA TOSHIROU
NAKAJIMA MASAHIRO
KONDOU SHIGEJI

Application Number:

JP7432883A

Publication Date:

November 10, 1984

Filing Date:

April 27, 1983

Export Citation:

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Assignee:

KAYAKU ANTIBIOTIC RESEARCH CO
KONDO SHIGEJI

International Classes:

C12P21/00; C07K1/14; C07K7/06; C07K14/005; C07K14/195; C07K14/41; C12P21/02; C12R1/645; (IPC1-7): C07G7/00; C12P21/00

Attorney, Agent or Firm:

Ariga Miyuki

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