PURPOSE: To obtain the titled compound by a simple device by easy operations industrially advantageously, by bringing a solution comprising vitamin B12 containing admixtures into contact with a divinylbenzene/styrene copolymer resin, eluting vitamin B12 with an elutant consisting of an aqueous solution of a lower ketone, etc.
CONSTITUTION: A product containing vitamin B12 obtaind by cultivating a strain of Propionibacterium shermanii IFO12391 capable of producing vitamin B12 is centrifuged, and filtered to give a mold. Vitamin B12 together with admixtures is extracted with water at 80°C from the mold, to give an aqueous solution of crude vitamin B12. The solution comprising vitamin B12 containing admixtures is brought into contact with a divinylbenzene/styrene copolymer resin, vitamin B12 is adsorbed on the resin, it is eluted with an elutant consisting of an aqueous solution of a compound such as a lower ketone, lower ester, lower ether, etc., and an active eluation fraction is collected to give the desired high-purity compound.
KOJIMA ICHIROU
OOGUCHI YUTAKA
Next Patent: JPS5995300