Title:
ＲＮＡ干渉を媒介する短鎖ＲＮＡ分子
Document Type and Number:
Japanese Patent JP5749892
Kind Code:
B2
Abstract:
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
Inventors:
Toushur, Thomas
Elvasher, Seida
Rendeckel, winfried
Wilm, Matthias
Ruhrmann, Reinhard
Elvasher, Seida
Rendeckel, winfried
Wilm, Matthias
Ruhrmann, Reinhard
Application Number:
JP2010046471A
Publication Date:
July 15, 2015
Filing Date:
March 03, 2010
Export Citation:
Assignee:
Max-Plank-Gezelle Shaft Tool Forderung Del Wissenschafften A.V.
European Molecular Biology Laboratory
European Molecular Biology Laboratory
International Classes:
A01K67/027; A01H5/00; C12N15/09; A61K31/7105; A61K45/00; A61K48/00; A61P31/12; A61P35/00; A61P37/02; A61P43/00; C07H21/00; C12N1/00; C12N5/10; C12N15/10; C12N15/113; C12P21/02; C12Q1/02; G01N33/15; G01N33/50; G01N33/68; A61K9/00; A61K38/00; C07H; C12N
Foreign References:
| WO2001075164A1 |
Other References:
Cell, 2000.04, Vol.101, p.235-238
Cell, 2000.03, Vol.101, p.25-33
Mol. Cell, 2000.11, Vol.6, p.1077-1087
ELBASHIR SAYDA M,GENES AND DEVELOPMENT,米国,COLD SPRING HARBOR LABORATORY PRESS,2001年 1月15日,V15 N2,P188-200
ZAMORE PHILLIP D,CELL,米国,CELL PRESS,2000年 3月31日,V101 N1,P25-33
BASS BRENDA L,CELL,米国,2000年 4月28日,V101 N3,P235-238
Cell, 2000.03, Vol.101, p.25-33
Mol. Cell, 2000.11, Vol.6, p.1077-1087
ELBASHIR SAYDA M,GENES AND DEVELOPMENT,米国,COLD SPRING HARBOR LABORATORY PRESS,2001年 1月15日,V15 N2,P188-200
ZAMORE PHILLIP D,CELL,米国,CELL PRESS,2000年 3月31日,V101 N1,P25-33
BASS BRENDA L,CELL,米国,2000年 4月28日,V101 N3,P235-238
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Eiichi Arai
Natsuo Tanaka
Satoshi Fujita
Eiichi Arai
Natsuo Tanaka