Title:
SPECIFICATION OF FUNCTIONAL CRANIAL PLACODE DERIVATIVES FROM HUMAN PLURIPOTENT STEM CELLS
Document Type and Number:
Japanese Patent JP2022123137
Kind Code:
A
Abstract:
To provide efficient derivation of cranial placodes from human pluripotent stem cells where the timed removal of a BMP inhibitor Noggin, a component of the dual-SMAD inhibition strategy of neural induction, triggers placode induction at the expense of CNS fates.SOLUTION: Cranial placodes are embryonic structures essential for sensory and endocrine organ development. Further fate specification at a pre-placode stage enables selective generation of placode-derived trigeminal ganglia capable of in vivo engraftment, mature lens fibers and anterior pituitary hormone-producing cells that upon transplantation produce hormones including, but not limited to, human growth hormone and adrenocorticotropic hormone in vivo. Alternatively, anterior pituitary hormone-producing cells are generated also in cell culture systems in vitro.SELECTED DRAWING: None
Inventors:
STUART CHAMBERS
LORENZ STUDER
ZEHRA DINCER
BASTIAN ZIMMER
LORENZ STUDER
ZEHRA DINCER
BASTIAN ZIMMER
Application Number:
JP2022101762A
Publication Date:
August 23, 2022
Filing Date:
June 24, 2022
Export Citation:
Assignee:
MEMORIAL SLOAN KETTERING CANCER CENTER
International Classes:
A61K35/12; C12N5/071; A61P5/00; A61P5/06; A61P25/00; A61P25/02; A61P25/06; A61P27/02; C12N5/10
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Morishita Natsuki