Title:
生体組織の滅菌
Document Type and Number:
Japanese Patent JP4619597
Kind Code:
B2
Abstract:
Allograft tissue prostheses, as well as tissue valves for replacement of defective heart valves and other tissue-engineered products (TEPs), are effectively sterilized by treatment with a coupling agent known to create amide linkages between amines and carboxylic acids in the presence of a protecting agent. The sterilization treatment preferably employs EDC as a water-soluble coupling agent, plus a water-soluble protecting agent which complexes with potentially reactive amine or carboxyl moieties on the biological tissue, in the optional presence of isopropanol or an equivalent alkanol. One preferred sterilization treatment process uses a buffered aqueous solution where a hydroxymonoamine buffer, such as TRIS plus ethanolamine, provides both the buffering effect and the protecting agents, effectively complexing with potentially reactive carboxyl moieties on the tissue. The treatment is particularly advantageous for sterilizing fresh biological tissue where substantial cross-linking that would alter physical properties of the tissue is undesirable or any tissues or TEPs where a controllable amount of cross-linking is desired.
Inventors:
Gene-Marie Giraldot
Marie-Nadia Girard Dot
Marie-Nadia Girard Dot
Application Number:
JP2001514758A
Publication Date:
January 26, 2011
Filing Date:
August 10, 2000
Export Citation:
Assignee:
Biomedical Design, Inc.
International Classes:
A01N1/00; A01N1/02; A61L2/00; A61L27/38
Domestic Patent References:
| JP60500014A |
Foreign References:
| WO1998034650A1 | ||||
| EP0897942A1 | ||||
| US5447536 | ||||
| WO1997027885A1 | ||||
| WO1995022361A1 |
Attorney, Agent or Firm:
Yoshikazu Tani
Kazuo Abe
Kazuo Abe