Title:
修飾バックボーンを有するアンチセンスプローブと核酸の液中ハイブリッド形成
Document Type and Number:
Japanese Patent JP4035589
Kind Code:
B2
Abstract:
The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a fluid medium using laser induced fluorescence of antisense probes. The probes can have anionic backbones of reduced negative charge. Suitable probes can include methylphosphonate backbones. When the hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the number of mismatches between the probe and target. When the hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence and proportional to the number of mismatches between the probe and target. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
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Inventors:
Knee, Aileen Jao-Fen
Wu, Yuan Min
Wu, Yuan Min
Application Number:
JP2000551045A
Publication Date:
January 23, 2008
Filing Date:
May 20, 1999
Export Citation:
Assignee:
Ingeneus Incorporated
International Classes:
C12N15/09; C12P19/34; C12Q1/68; C12Q1/6816
Foreign References:
| WO1997012995A1 | ||||
| WO1998038334A1 |
Other References:
Nature Biotechnology,1996年,Vol.14,pp.1700-1704
Nature Biotechnology,1996年,Vol.14,p.247
Nature Biotechnology,1996年,Vol.14,p.247
Attorney, Agent or Firm:
Kazuo Shamoto
Shinjiro Ono
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Akiko Yamaguchi
Shinjiro Ono
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Akiko Yamaguchi