To provide a substrate for hybridization where surface coverage rate and activity are improved and DNA chains are immobilized on the sub strate surface, to provide a manufacturing method of the substrate, and further to provide a complementary testing method using the substrate.
In the substrate for hybridization, a DNA chain having a double chain section and a single chain section is immobilized on the surface of a substrate, and the double change section side of the DNA chain is immobilized to the substrate surface. In the manufacturing method of the substrate, a double chain section and a single chain section are provided on the metal surface of a metal substrate or a substrate having a metal covering, a DNA chain having a thiol group is brought into contact with the end of the double chain section, and the DNA chain is immobilized on the metal surface. A method for testing the complementality of a target DNA with the single chain section of the DNA chain which has the target DNA, a double chain section and a single chain section by bringing the target DNA into contact with the surface where the DNA chain on the substrate is immobilized, is also provided.
HARA MASAHIKO
NAKAJIMA TAKESHI
ITO EISUKE
